DOT authorizes any individual who has received training specified in 49 CFR § 40.33 to act as a urine collector, and in § 40.35 to act as an oral fluid collector, except in the following situations:

  1. The immediate supervisor of a particular employee must not act as the collector when that employee is tested
  2. An employee who is in a safety-sensitive position and is subject to the DOT drug testing rules should not be a collector (or an observer or monitor) for co-workers who are in the same testing pool or who work together with that employee on a daily basis
  3. The employee must not be the collector of his or her own specimen
  4. A collector must not be related to the employee being tested (e.g., spouse, ex-spouse, relative) or a close personal friend